Tutorial Videos


Generating a Compensation Matrix using CytoFLEX Duration: 3 minutes Step-by-step instructions for generating a compensation matrix in CytExpert for CytoFLEX, the acquisition and analysis software used in the CytoFLEX Flow Cytometry platform. Tháng
CytoFLEX How to Set Up Violet Side Scatter Duration: xx minutes (x Videos at x minutes each) Tháng
EMnetik System Product Tutorial Duration: 12 minutes 32 seconds Watch this EMnetik System tutorial video focusing on PCR cleanup and plasmid purification. Tháng
How to Install Rotors on Allegra V-15R Duration: 1 minute 2 seconds Installing a rotor into the Allegra V-15R benchtop centrifuge is simple. Watch this video to learn how. Tháng
How to Program ECO Mode on Allegra V-15R Duration: 1 minute 18 seconds Watch this video to learn how ECO Mode works and how to set it up. Tháng
How to Program Rapid Temp on Allegra V-15R Duration: 0 minutes 59 seconds Watch this video to learn how Rapid Temp works and how to set it up. Tháng
How to Register Your Instrument with BeckmanConnect Duration: 3 minutes 40 seconds In this short video you will learn how to install the BeckmanConnect remote diagnostic software on your Beckman Coulter instrument. Tháng
Kaluza Cell Cycle Analysis Tutorial with Dr. David Onion Duration: 12 minutes 30 seconds Flow cytometry is an excellent way to measure DNA content because of its high sensitivity high precision acquisition of cell by cell total fluorescence, and of course DNA content analysis was the original fluorescent application of flow cytometry looking for an aneuploidy in cancer specimens. However, now-a-days the most common reason for measuring DNA content is because it gives us a very nice way to profile the position of cells within the cell cycle. Let's explore the cell cycle analysis plot in Kaluza Analysis Software together with Dr. David Onion. Tháng
Kaluza Composites Tutorial with Dr. David Onion Duration: 5 minutes 12 seconds The Composite feature in Kaluza Analysis will allow you to visualize multiple files in the same plot or report sheet, and enable you to create an overlay plot. Overlay plots are a powerful visual to present your data, allowing you to highlight small differences or demonstrate data sets to be very similar. Tháng
Duration: xx minutes (x Videos at x minutes each) Tháng
Kaluza Linked Gates Tutorial with Dr. David Onion Duration: xx minutes (x Videos at x minutes each) Linked Gate feature is a useful tool in Kaluza Analysis Software that helps ensure consistency when placing gates that you want to be identical on multiple bivariate plots or indeed on histograms. Tháng
Cytobank CITRUS Duration: 58 minutes CITRUS (cluster identification, characterization, and regression) is an algorithm designed for the fully automated discovery of statistically significant stratifying biological signatures. Input data includes single cell datasets containing numerous samples across multiple known endpoints (e.g., responders versus non-responders). This video covers the basics of CITRUS within Cytobank, advanced applications of CITRUS, and a brief Q&A session. Tháng
Cytobank Experiment Manager Duration: 10 minutes The Cytobank platform utilizes the experiment manager to organize data, analysis, and results. The experiment manager facilitates collaboration across labs, institutions, and the scientific community. This video covers the features of the experiment manager in detail. Tháng
Cytobank FlowSOM Duration: 47 minutes FlowSOM is a clustering algorithm that speeds time to analysis and quality of clustering with Self-Organizing Maps (SOMs) that can reveal how all markers are behaving on all cells and can detect subsets that might otherwise be missed. It clusters cells (or other observations) based on chosen clustering channels (or markers/features), generates a SOM of clusters, produces a Minimum Spanning Tree (MST) of the clusters, and assigns each cluster to a metacluster, effectively grouping them into a population. The FlowSOM algorithm outputs SOMs and MSTs showing population abundances and marker expression in various formats including pie charts, star plots, and channel-colored plots. This video covers the background of FlowSOM, how to configure a FlowSOM run within Cytobank, and how to interpret the results. Tháng
Cytobank viSNE Duration: 36 minutes viSNE is a dimensionality reduction algorithm that reduces high-parameter data down to two dimensions for rapid exploratory data analysis of any data type. The output is a set of two new parameters, viSNE 1 and viSNE 2. These parameters can be used to create a viSNE map that can be color-coded by channel to allow for easy visualization of highly complex data. This video covers the background of viSNE, how to configure a viSNE run within Cytobank, and how to interpret the results. Tháng
Creating an Experiment using CytoFLEX Duration: 5 minutes Step-by-step instructions for creating an Experiment for multicolor flow cytometry in CytExpert for CytoFLEX, the acquisition and analysis software used in the CytoFLEX Flow Cytometry platform Tháng
How to Set-Up and Run a Program on Allegra V-15R Duration: 1 minutes 13 seconds Up to 50 run programs can be stored in the Allegra V-15R benchtop centrifuge. Watch this video to learn how to save programs and run them on your centrifuge. Tháng
Kaluza Batch Analysis Tutorial with Dr. David Onion Duration: 7 minutes 42 seconds Exploring batch analysis with Kaluza Analysis Software. In a nutshell batch analysis allows you to use any protocol file, composite, or Kaluza analysis file as a template to perform analysis on as many files as you like. This would become really useful if you were compiling reports, perhaps you’re writing a paper and you wanted to get all your data together and make sure it is analyzed in exactly the same way. Tháng
Kaluza Boolean Gates tutorial with Dr. David Onion Duration: 6 minutes 49 seconds Boolean gating allows us to create gates based on a logical selection of other individual gates and with the use of operators such as AND, NOT, and OR. You might want to create a simple combination of 2 gates, right up to a complex selection of multiple gates including or excluding populations as required. Tháng
Setting up Statistics with Kaluza Software Tutorial Duration: 45 minutes 49 seconds Comprehensive overview of several advanced analysis features of Kaluza Analysis software for manipulating larger sets of data. Tháng
How to Fill and Seal a Quick-Seal Tube Duration: 2 minutes 32 seconds A video on filling and sealing Beckman Coulter’s Quick-Seal tubes. Tháng
How to Fill and Plug a OptiSeal Tube Duration: 1 minute 22 seconds A video on filling and plugging Beckman Coulter’s OptiSeal tubes Tháng
Syringe Extraction of a Ultra-Clear, Quick-Seal Tube Duration: 1 minute 8 seconds A video on syringe extraction, when using Beckman Coulter’s Ultra-Clear, Quick-Seal tubes. Tháng
5 Years of high-throughput strain engineering with the Echo: lessons from Zymergen Automation presented by Christopher Bremner, Automation Engineer, Zymergen @SLAS2020 Duration: 19 minutes 14 seconds Zymergen has used the Echo Liquid Handler to automate strain engineering for many years now. In this talk, Chris reflects on some lessons learned during early Echo usage, provides some tips to maximize throughput of your Echo device, and showcases how Zymergen currently use Echo devices in their workflows. Tháng
Characterization of CytoFLEX Gain Settings Duration: Characterizaiton of CytoFLEX Gain Settings Tháng
Cytobank Basics Duration: 9 minutes 35 seconds The Cytobank platform is a cloud-based platform that accelerates research productivity by enabling you to analyze and visualize multiple single-cell data sets simultaneously. This video covers the experiment manager, experiment summary, working illustration, exporting statistics, and sharing and managing your experiments within Cytobank. Tháng
Kaluza Levey-Jennings Plots and Quality Control Analysis Tutorial with Dr. David Onion Duration: 8 minutes 52 seconds Consistency and accuracy of data is key to flow cytometry and so we often want to ensure this through some sort of quality control procedure. It might be routine QC of the machines through manufacture recommend controls or standards or you may wish to assess the reproducibility of a new staining panel that you are developing and so run a series of repeats on identical samples. Either way a Quality Control report gives you a quick way to analyze the data and spot any problems and outliers through the use of Levey-Jennings plots. Tháng
Kaluza Radar and Tree Plots Tutorial with Dr. David Onion Duration: 11 minutes 32 seconds Radar and Tree plots allow you in Kaluza Analysis Software to visualize your data. Both of these plot types address the problem of understanding and visualizing the multi-dimensional data which is becoming ever more important as we begin to utilize more and more parameters as standard in our flow cytometry. They are particularly useful in dissecting multi-parameter phenotyping data and they can also help us to understand the relationship between multiple functional markers such as cytokines or phosphorylation events. Tháng
Off-the-shelf, Echo-based DNA Assembly Automation presented by George McArthur, Arzeda @SLAS2020 Duration: 16 minutes 36 seconds In this talk, George describes how his team transformed a series of arduous, complex, time-consuming and error-prone laboratory tasks into an effortless, straightforward, fast and mistake-free workflow using a combination of accessible software and hardware automation. Tháng
Seminar: Diverse Applications of Flow Cytometry to Answer Biological Questions Duration: 43 minutes 58 seconds Karen Hogg from the University of York Biological Technology Facility describes the diverse applications of flow cytometry that her team executes on behalf of the university’s researchers. Flow cytometry has moved beyond traditional blood cell immunophenotyping. This presentation at CYTO 2016 features work done with the CytoFLEX flow cytometer to understand cell heterogeneity and behavior on samples as diverse as bacteria, yeast, pollen and plant cells. Tháng
Extended Features for Kaluza Software Tutorial Duration: 48 minutes 46 seconds This webinar covers the many extended features of Kaluza Analysis software including the Gallios simulator. Includes online demos. Tháng
Getting to Know Kaluza Acquisition Software Tutorial Duration: 49 minutes 10 seconds This webinar provides an overview of Kaluza for Gallios Acquisition software highlighting how easy Kaluza is to use. Tháng
Seminar: High Dimensional Flow Cytometry on Small Instrument Platforms Tutorial Duration: 21 minutes 4 seconds William Telford from the NIH describes biological applications using yellow green and near UV laser excitation sources available on the CytoFLEX S flow cytometer. He provides an overview of the CytoFLEX technology and how it contributes to the results obtained on biological samples using high dimensional analysis to identify very rare populations. Tháng
Kaluza Analysis Overview Tutorial Duration: 6 minutes 43 seconds Provides an overview of the major functions of Kaluza Analysis Software, from loading plots to unique plot types. Tháng
Kaluza for Gallios Compensation Worklist Tutorial Duration: 2 minutes 3 seconds Follow this online demo to learn how to create a compensation matrix (Kaluza Analysis 1.3). Tháng
Kaluza for Gallios Simulator with a Legacy Browser Tutorial Duration: 2 minutes 7 seconds Watch this online demo to learn how to identify Gallios configuration, create a new carousel, add/delete/change .FCS files and change parameter sources. Tháng
Kaluza for Gallios Software Tutorial - Flow Cytometry Duration: 9 minutes 30 seconds This tutorial cover variety of tasks in Kaluza including using the simulator, creating a new carousel, adding/deleting .FCS files to the carousel, changing an active carousel, changing parameter sources, creating a compensation matrix and creating a compensation worklist. Tháng
Kaluza Analysis Software Tutorial - Loading Files and Creating Plots Duration: 3 minutes 31 seconds Kaluza Analysis tutorial on getting started with the software including demonstration of uploading flow cytometry FCS files and creating plots and histograms. Shows how to access functions using the ribbon and radial menus. Tháng
Kaluza Analysis Software: Creating Overlay Plots Duration: 3 minutes 32 seconds Learn more about how to set up an overlay plot, add/remove and replace histograms, and how to use various formatting features. Tháng
Optima AUC Cell Loading Tutorial Duration: 2 minutes Tháng English, Japanese
Intellifuge Calculator Introduction Duration: 1 minute 48 seconds Introducing the new Intellifuge Calculator that helps you calculate, convert and configure with ease. Tháng
Optima AUC Cell Assembly Tutorial Duration: 2 minutes Tháng Japanese, English
Optima AUC Cell Preparation Tutorial Duration: 2 minutes Tháng Japanese
Cytobank SPADE Duration: 6 minutes SPADE stands for "Spanning-tree Progression Analysis of Density-normalized Events." SPADE clusters phenotypically similar cells into a hierarchy that allows high-throughput, multidimensional analysis of heterogeneous samples. This tutorial shows how to run a SPADE analysis on the Cytobank platform. Tháng

Generating a Compensation Matrix using CytoFLEX

Duration: 3 minutes

Step-by-step instructions for generating a compensation matrix in CytExpert for CytoFLEX, the acquisition and analysis software used in the CytoFLEX Flow Cytometry platform.

Tháng

CytoFLEX How to Set Up Violet Side Scatter

Duration: xx minutes (x Videos at x minutes each)

Tháng

EMnetik System Product Tutorial

Duration: 12 minutes 32 seconds Watch this EMnetik System tutorial video focusing on PCR cleanup and plasmid purification.

Tháng

How to Install Rotors on Allegra V-15R

Duration: 1 minute 2 seconds Installing a rotor into the Allegra V-15R benchtop centrifuge is simple. Watch this video to learn how.

Tháng

How to Program ECO Mode on Allegra V-15R

Duration: 1 minute 18 seconds Watch this video to learn how ECO Mode works and how to set it up.

Tháng

How to Program Rapid Temp on Allegra V-15R

Duration: 0 minutes 59 seconds Watch this video to learn how Rapid Temp works and how to set it up.

Tháng

How to Register Your Instrument with BeckmanConnect

Duration: 3 minutes 40 seconds In this short video you will learn how to install the BeckmanConnect remote diagnostic software on your Beckman Coulter instrument.

Tháng

Kaluza Cell Cycle Analysis Tutorial with Dr. David Onion

Duration: 12 minutes 30 seconds

Flow cytometry is an excellent way to measure DNA content because of its high sensitivity high precision acquisition of cell by cell total fluorescence, and of course DNA content analysis was the original fluorescent application of flow cytometry looking for an aneuploidy in cancer specimens. However, now-a-days the most common reason for measuring DNA content is because it gives us a very nice way to profile the position of cells within the cell cycle. Let's explore the cell cycle analysis plot in Kaluza Analysis Software together with Dr. David Onion.

Tháng