EMnetik PCR System Performance Data

PCR Cleanup - Simplified

~2x faster turnaround time compared to column cleanups (16 min vs 30 min)
>80% recovery (comparable to column cleanup kits)
Significantly fewer touchpoints (< 50 touchpoints compared to 300 for columns)
- No need to handle small columns or use a single-channel pipette
- Move samples from a thermocycler to the EMnetik 24 system, and don’t move them again until final elution
Intuitive user interface removes guesswork by providing clear, step-by-step instructions

Choose your sample input, elution and labware.

Genomics EMnetik PCR Performance Figure 1

Figure 1

These graphs demonstrate your flexible options.
Figure 1 left: Yield of elutions from 2 different starting inputs and 2 different elution volume options (50 µL and 20 µL). Bars indicate the average of 9 replicates on the device; error bars indicate the standard deviation of the replicates. Figure 1 right: Yield does not vary widely when using different labware. The first three bars show the yield using PCR 8-strip tubes, and the second three bars show the yield using single PCR tubes. The bars represent the average of 8 samples; error bars indicate the standard deviation of the 8 samples. Tubes are as follows: A: Thermo Scientific AB-2005, B:VWR 93001-118, C: VWR 20170-002, D: Thermo Scientific AB-0337, E: VWR 20170-010, and F: VWR 20170-012.

DNA is stable in the spinning magnetic beads.

Genomics EMnetik PCR Performance Figure 2

Figure 2

Figure 2: To test that DNA was not degraded during the automatic bead mixing or separation, a ladder was used as input lanes; 1 and 2 show the input ladder and lanes 3 - 8 show the ladder after cleanup. All bands can be seen in all lanes, indicating that DNA is not degraded during the cleanup process.