Rôto máy siêu ly tâm phân tích
Rôto máy siêu ly tâm phân tích (AUC) độc đáo ở chỗ chúng phải cho phép tia sáng đi qua mẫu đang quay. Các hệ thống quang học trong AUC (Độ hấp thụ UV-Vis và giao thoa Rayleigh) quan sát trạng thái dung dịch của các phân tử khi chúng trải qua quá trình lắng.
Rôto máy siêu ly tâm phân tích chỉ được sử dụng trong Optima AUC hoặc ProteomeLab XL-A/I.
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FAQ on Analytical Ultracentrifuge Rotors
What is analytical ultracentrifugation (AUC)?
Analytical ultracentrifugation (AUC) is a technique that combines high-speed centrifugation with optical detection to study the behavior of molecules in solution. Researchers use AUC to characterize biomolecules, determine molecular weight, and analyze molecular interactions without modifying the sample.
Learn MoreWhat applications is analytical ultracentrifugation used for?
Analytical ultracentrifugation is commonly used for protein characterization, molecular weight determination, aggregation analysis, biologics development, viral vector characterization and studies of macromolecular interactions.
How does analytical ultracentrifugation work?
In analytical ultracentrifugation, samples are subjected to high centrifugal forces while optical systems continuously monitor molecular movement during sedimentation. The resulting data can be used to evaluate molecular size shape, heterogeneity, and interactions.
What is sedimentation equilibrium (SE-AUC)?
Sedimentation equilibrium is an AUC technique in which sedimentation is balanced by diffusion until equilibrium is reached. Researchers commonly use SE-AUC to determine molecular weight and study molecular association behavior in solution.
Why is analytical ultracentrifugation used for protein characterization?
AUC enables proteins to be analyzed in their native solution state without labeling or immobilization. This provides information about molecular weight, oligomerization, aggregation and sample heterogeneity that can be difficult to obtain using other analytical techniques.
What is sedimentation velocity (SV-AUC)?
Sedimentation velocity analytical ultracentrifugation measures how quickly particles move through solution when exposed to centrifugal force. SV-AUC is commonly used to assess size distributions, aggregation levels, sample purity and molecular interactions.
What is the difference between analytical and preparative ultracentrifugation?
Preparative ultracentrifugation is used to isolate, purify or concentrate biological materials. Analytical ultracentrifugation is used to characterize molecules and study their physical properties in solution. Rather than recovering material, AUC generates data that provide insights into molecular behavior and interactions.
